DNA Topoisomerase Protocols Volume 1: DNA Topology and Enzymes (Methods in Molecular Biology Vol 94)
Bjornsti, Mary-Ann, Osheroff, Neil
DNA Topoisomerase Protocols is an unprecedented collection of cutting-edge experimental protocols for investigating DNA structure, topology, and DNA topoisomerase function. Described by expert experimentalists who have perfected the techniques, these time-proven methods utilize new approaches to study changes in DNA topology (linking number, knotting, catenation, and relaxation) and DNA structure (bending), as well as to assess chromosome structure through FLP-mediated recombination and to analyze bacterial nucleoid structure. State-of-the-art protocols also detail the expression and purification of bacterial, viral, and eukaryotic DNA topoisomerases from a variety of sources, including baculovirus, bacterial, yeast, and mammalian expression systems.
หมวดหมู่:
ปี:
2014
สำนักพิมพ์:
Springer
ภาษา:
english
ISBN 10:
0896034445
ISBN 13:
9780896034440
ไฟล์:
EPUB, 4.82 MB
IPFS:
,
english, 2014
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